Easy To Use Patents Search & Patent Lawyer Directory

At Patents you can conduct a Patent Search, File a Patent Application, find a Patent Attorney, or search available technology through our Patent Exchange. Patents are available using simple keyword or date criteria. If you are looking to hire a patent attorney, you've come to the right place. Protect your idea and hire a patent lawyer.


Search All Patents:



  This Patent May Be For Sale or Lease. Contact Us

  Is This Your Patent? Claim This Patent Now.



Register or Login To Download This Patent As A PDF




United States Patent Application 20170354621
Kind Code A1
WU; Yi Juang ;   et al. December 14, 2017

SULFONAMIDE PHARMACEUTICAL COMPOSITION

Abstract

A sulfonamide pharmaceutical composition. The present invention relates to a sulfonamide compound injectable preparation comprising a sulfonamide compound or a derivative thereof. The injectable preparation is prepared from the sulfonamide compound and a pharmaceutically acceptable carrier through certain preparation technologies. The sulfonamide compound injectable preparation involved in the present invention is stable and controllable in quality and effective.


Inventors: WU; Yi Juang; (Tianjin, CN) ; YAO; Xiaoqing; (Tianjin, CN) ; SUN; Changhai; (Tianjin, CN) ; TIAN; Li; (Tianjin, CN) ; ZHAO; Xinying; (Tianjin, CN) ; HAN; Zhidong; (Tianjin, CN) ; LIN; Chuangyu; (Tianjin, CN)
Applicant:
Name City State Country Type

TIANJIN HONGRI JIAN DA KANG MEDICAL TECHNOLOGY CO., LTD

Tianjin

CN
Family ID: 1000002835234
Appl. No.: 15/541776
Filed: January 4, 2016
PCT Filed: January 4, 2016
PCT NO: PCT/CN2016/070015
371 Date: July 6, 2017


Current U.S. Class: 1/1
Current CPC Class: A61K 31/18 20130101; A61K 47/10 20130101; A61K 9/0019 20130101; A61K 47/20 20130101; A61K 47/12 20130101
International Class: A61K 31/18 20060101 A61K031/18; A61K 9/00 20060101 A61K009/00; A61K 47/20 20060101 A61K047/20; A61K 47/12 20060101 A61K047/12; A61K 47/10 20060101 A61K047/10

Foreign Application Data

DateCodeApplication Number
Jan 6, 2015CN2015100059955

Claims



1. A sulfonamide pharmaceutical composition, prepared from the following raw materials: TABLE-US-00017 Raw materials Percentage by weight sulfonamide compound 20%-40% PEG-400 20%-40% 1,2-propanediol 5%-10% Sebacic acid 2%-5% 2-ethyl-1,3-hexanediol 10%-20% Dimethyl sulfoxide .sup. 0-10% Anhydrous ethanol .sup. 0-10%.

2. The sulfonamide pharmaceutical composition of claim 1, wherein the sulfonamide compound is one, or a mixture of two or more, at any ratio, of the following: ##STR00007## wherein R.dbd.H, C.sub.2H.sub.5, or ##STR00008##

3. The sulfonamide pharmaceutical composition of claim 1, wherein the sulfonamide compound is: ##STR00009##

4. The sulfonamide pharmaceutical composition of claim 1, prepared from the following raw materials: TABLE-US-00018 Raw materials Percentage by weight p-Toluenesulfonamide 20%-40% PEG-400 20%-40% 1,2-propanediol 5%-10% Sebacic acid 2%-5% 2-ethyl-1,3-hexanediol 10%-20% Dimethyl sulfoxide .sup. 0-10% Anhydrous ethanol .sup. 0-10%.

5. The sulfonamide pharmaceutical composition of claim 1, prepared from the following raw materials: TABLE-US-00019 Raw materials Percentage by weight p-Toluenesulfonamide 30% PEG-400 30% 1,2-propanediol 8% Sabacic acid 4% 2-ethyl-1,3-hexanediol 15% Dimethyl sulfoxide 5% Anhydrous ethanol 8%.

6. The sulfonamide pharmaceutical composition of claim 1, wherein said pharmaceutical composition is injectable.

7. A method to prepare the sulfonamide pharmaceutical composition of claim 1, comprising the steps of: 1) Putting a prescription amount of sulfonamide compound, PEG-400, and 2-ethyl-1,3-hexanediol into a container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution A, for later use; 2) Putting a prescription amount of sebacic acid and 1,2-propanediol, into a separate container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution B, for later use; 3) Mixing Solution A and Solution B while maintaining the temperature at 85.degree. C.-95.degree. C., and stirring to obtain a homogenous solution for later use; 4) Putting a prescription amount of dimethyl sulfoxide and a small amount of anhydrous ethanol into a container, stirring and mixing well to obtain a homogenous solution, Solution C, for later use; 5) Cooling the mixture of Solution A and Solution B to 60.degree. C., adding Solution C to the mixture, stirring and mixing well before cooling down to room temperature, adding the remaining amount of anhydrous ethanol and mixing well; 6) Filtering with 0.45 um microporous membrane, aliquoting into 5 ml ampoules and sealing the ampoules; and 7) Sterilizing at 121.degree. C. for 30 minutes.

8. A method to prepare the sulfonamide pharmaceutical composition of claim 1 from the following raw materials, comprising the steps of: TABLE-US-00020 Raw materials Percentage by weight p-Toluenesulfonamide 30% PEG-400 30% 1,2-propanediol 8% Sebacic acid 4% 2-ethyl-1,3-hexanediol 15% Dimethyl sulfoxide 5% Anhydrous ethanol 8%

1) Putting a prescription amount of sulfonamide compound, PEG-400, and 2-ethyl-1,3-hexanediol into a container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution A, for later use; 2) Putting a prescription amount of sebacic acid and 1,2-propanediol, into a separate container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution B, for later use; 3) Mixing Solution A and Solution B while maintaining the temperature at 85.degree. C.-95.degree. C., and stirring well to obtain a homogenous solution for later use; 4) Putting a prescription amount of dimethyl sulfoxide and a small amount of anhydrous ethanol into a container, stirring and mixing well to obtain a homogenous solution, Solution C, for later use; 5) Cooling the mixture of Solution A and Solution B to 60.degree. C., adding Solution C to the mixture, stirring and mixing well before cooling down to room temperature, adding the remaining amount of anhydrous ethanol and mixing well; 6) Filtering with 0.45 um microporous membrane, aliquoting into 5 ml ampoules and sealing the ampoules; and 7) Sterilizing at 121.degree. C. for 30 minutes.

9-10. (canceled)

11. A method to treat cancer in a subject, comprising administering the sulfonamide pharmaceutical composition of claim 1 to said subject.

12. The method of claim 11, wherein the cancer is central type lung cancer.
Description



FIELD OF THE INVENTION

[0001] This invention relates to a sulfonamide pharmaceutical composition, and their preparation and use in the pharmaceutical industry.

BACKGROUND OF THE INVENTION

[0002] Cancer is a common disease in the world today. Most cancers are diagnosed at late stage and the cure rate is low. Most of the current anticancer drugs have toxic side effects including hair loss, vomiting, decrease of white blood cell count, bone marrow suppression, and decrease in immune system function. The major reason is that these drugs act on part of the cell metabolic cycle, and they do not selectively target cancer cells as opposed to healthy cells. When they kill cancer cells, they also damage healthy cells, especially those healthy cells that are undergoing rapid metabolism.

[0003] Sulfonamide compounds have been clinically used as an antimicrobial for decades. Today, they are still one of the most commonly used antimicrobials, second only to antibiotics because of their broad antibacterial spectrum, stability, ease of use, and low price. As more in-depth studies are conducted, researchers have found that sulfonamide compounds have a wider range of biological activities, including its diuretic effect, anti-thyroid effect, anti-diabetic effect, anti-hypoglycemic effect, and its ability to treat cataract. In recent years, a large number of sulfonamide compounds with anti-tumor activity have been reported, and many among them have entered clinical trials. These compounds act on different molecular targets and exhibit significant biological activity. Some compounds exhibit high selectivity and specificity for different targets.

[0004] The mechanisms for the actions of these sulfonamide compounds are diverse, including disrupting tubulin polymerization, blocking normal cell cycle, inhibiting carbonic anhydrase, inhibiting folic acid dependent enzymes, inhibiting methionine aminopeptidase and histone deacetylase, and inhibiting vascular endothelial cell growth factor and so on.

[0005] Chinese Patent ZL97108988.4 disclosed the formulation and method to prepare injections of sulfonamide compounds.

[0006] The compounds include:

##STR00001##

wherein R.dbd.H, C.sub.2H.sub.5, or

##STR00002##

[0007] Preferably, sulfonamide compounds are selected from:

##STR00003##

[0008] The formulation is as follows:

TABLE-US-00001 Said sulfonamide compound 10%~80% PEG-400 10%~60% 1,2-propanediol 5%~30% Suberic acid 1%~20% p-Toluenesulfonic acid 1%~15% Dimethyl sulfoxide 0%~20% Ethanol 0%~20%.

[0009] When using the above formulation to prepare p-toluenesulfonamide injections, it was accidently discovered that the above formulation has low stability, tends to crystallize after long storage time, and it also causes severe irritation to the injection site.

[0010] Studies on the formulation and preparation method above showed that the problems are caused by suberic acid and p-toluenesulfonic acid. This invention effectively solved the problems by replacing suberic acid with sebacic acid, and replacing p-toluenesulfonic acid with 2-ethyl-1,3-hexanediol.

DETAILED DESCRIPTION OF THE INVENTION

[0011] The present invention solves the problems of low solubility and ease of crystallization associated with known injection formulations by improving the formulation and method to prepare injections of sulfonamide compounds. Comparative toxicity test also shows that the present formulation is less toxic and safer for clinical use.

[0012] The goal of this invention is to provide an injection formulation comprising a sulfonamide compound, made with the following raw materials:

TABLE-US-00002 Raw Materials Percentage by weight Sulfonamide compound 20%-40% PEG-400 20%-40% 1,2-propanediol 5%-10% Sebacic acid 2%-5% 2-ethyl-1,3-hexanediol 10%-20% Dimethyl sulfoxide .sup. 0-10% Anhydrous ethanol .sup. 0-10%.

[0013] Among polyethylene glycol-400 (PEG-400), 1,2-propanediol, sebacic acid, 2-ethyl-1,3-hexanediol, dimethyl sulfoxide and ethanol, only dimethyl sulfoxide and ethanol are dispensable, others are required.

[0014] The sulfonamide compound is selected from one, or a mixture of two or more, at any ratio, of the following:

##STR00004##

wherein R.dbd.H, C.sub.2H.sub.5, or

##STR00005##

[0015] Preferably, the sulfonamide compound is selected from:

##STR00006##

[0016] Preferably, the injection formulation of the present invention is prepared from the following raw materials:

TABLE-US-00003 Raw materials Percentage by weight P-Toluenesulfonamide 20-40% PEG-400 20-40% 1,2-propanediol 5-10% Sebacic acid 2-5% 2-ethyl-1,3-hexanediol 10-20% Dimethyl sulfoxide 0-10% Anhydrous ethanol 0-10%

[0017] Further preferred, the injection formulation of this invention is prepared from the following raw materials:

TABLE-US-00004 Raw materials Percentage by weight p-Toluenesulfonamide 30% PEG-400 30% 1,2-propanediol 8% Sebacic acid 4% 2-ethyl-1,3-hexanediol 15% Dimethyl sulfoxide 5% Anhydrous ethanol 8%.

[0018] The present invention improves upon the known injection formulations by removing suberic acid and adding the solubilizer 2-ethyl-1,3-hexanediol. The combination of PEG-400, 2-ethyl-1,3-hexanediol, and 1,2-propanediol can decrease the amount of 1,2-propanediol in the injection formulation, thereby decreasing irritation at the injection site.

[0019] Another goal of this invention is to provide a method to prepare the injection.

[0020] The preparation of the injection of this invention comprises the following steps: [0021] 1) Putting a prescription amount of sulfonamide compound, PEG-400, and 2-ethyl-1,3-hexanediol into a container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution A, for later use; [0022] 2) Putting a prescription amount of sebacic acid and 1,2-propanediol, into a separate container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution B, for later use; [0023] 3) Mixing Solution A and Solution B while maintaining the temperature at 85.degree. C.-95.degree. C., and stirring to obtain a homogenous solution for later use; [0024] 4) Putting a prescription amount of dimethyl sulfoxide and a small amount of anhydrous ethanol into a container, stirring and mixing well to obtain a homogenous solution, Solution C, for later use; [0025] 5) Cooling the mixture of Solution A and Solution B to 60.degree. C., adding Solution C to the mixture, stirring and mixing well before cooling down to room temperature, adding the remaining amount of anhydrous ethanol and mixing well; [0026] 6) Filtering with 0.45 um microporous membrane, aliquoting into 5 ml ampoules and sealing the ampoules; and [0027] 7) Sterilizing at 121.degree. C. for 30 minutes.

[0028] The beneficial effects of the present invention are further illustrated by the following experiments.

TABLE-US-00005 TABLE 1 Comparison of the formulation of the present invention with that disclosed in CN1073415C The Present Invention Example 2 in CN1073415C p-Toluenesulfonamide 30% p-Toluenesulfonamide 30% PEG-400 30% PEG-400 33.5% 1,2-propanediol 8% 1,2-propanediol 16.4% Sebacic acid 4% Suberic acid 8.2% 2-ethyl-1,3-hexanediol 15% p-Toluenesulfonic acid 3.7% Dimethyl sulfoxide 5% Dimethyl sulfoxide 6.7% Anhydrous ethanol 8% Ethanol 1.5%

[0029] The present invention is an improvement on the basis of the prior art (CN1073415C). As shown in the above table, the formulation of the present invention made the following improvements as compared to the formulation disclosed in Example 2 of the Chinese patent CN1073415C: [0030] 1. Use of sebacic acid instead of suberic acid, [0031] 2. Use of 2-ethyl-1,3-hexanediol instead of p-toluenesulfonic acid.

[0032] By making the above improvements, this invention solved a range of issues associated with the known formulation such as low stability.

[0033] The beneficial effects of the present invention are further illustrated by following studies.

Experiment 1. Study on the Chinese Patent CN1073415C

[0034] Comparative studies were conducted on injections prepared according to the examples disclosed in the CN1073415C patent.

TABLE-US-00006 Raw materials Example 1 Example 2 p-Toluenesulfonamide 30% 40% PEG-400 33.5% 40% 1,2-propanediol 16.4% 10% Suberic acid 8.2% 3% p-Toluenesulfonic acid 3.7% 2% Dimethyl sulfoxide 6.7% 3% Ethanol 1.5% 2% Stability Stored at Stored at 4.degree. C. .+-. 2.degree. C. for 4.degree. C. .+-. 2.degree. C. for 10 days, 10 days, crystals crystals precipitated precipitated LD50 12.54 mg/kg 11.30 mg/kg

[0035] Results show that crystals precipitated when the injection was refrigerated for a period of time, and this seriously affects the stability of the injection. After discovering the above problems, the inventors of this invention conducted research on the formulation and have found that the decrease in stability is likely to be caused by the presence of suberic acid and p-Toluenesulfonic acid in the formulation, for the following reasons: [0036] The effect of PEG-400 in the formulation is as follows: acting as a water-soluble solvent to increase the compatibility of the injection with the tissues in the body. [0037] The effect of 1,2-propanediol in the formulation is as follow: acting as a water-soluble solvent, to increase the compatibility of the injection with the tissues in the body and inhibiting crystallization. [0038] The effect of suberic acid in the formulation is as follows: it is a binary fatty acid and act as a fat-soluble solvent to increase the solubility of p-Toluenesulfonamide in the mixed solvent of the injection and to inhibit crystallization. [0039] The effect of p-Toluenesulfonic acid in the formulation is as follows: it is an aromatic sulfonic acid compound, to increase the solubility of p-Toluenesulfonamide in the mixed solvent of the injection, and to inhibit crystallization. [0040] The effect of dimethyl sulfoxide in the formulation is as follows: acting as an amphiphilic solvent to increase the solubility of p-Toluenesulfonamide in the mixed solvent of the injection, and to inhibit crystallization. [0041] The effect of ethanol in the formulation is as follows: acting as an amphiphilic solvent to increase the solubility of p-Toluenesulfonamide in the mixed solvent of the injection, and to adjust the overall volume of the solution due to its good fluidity.

[0042] The inventors conducted the following experiments to further validate the above conclusions.

[0043] The above problems still exist when the inventor adjusted the amount of other raw materials while keeping the amount of suberic acid and p-Toluenesulfonic acid constant.

TABLE-US-00007 Raw materials Formula 1 Formula 2 Formula 3 Formula 4 p-Toluenesulfonamide 30% 40% 30% 40% PEG-400 33.5% 40% / / PEG-200 / / 33.5% 40% 1,2-propanediol 16.4% 10% / / Glycerin / / 16.4% 10% Suberic acid 8.2% 3% 8.2% 3% p-Toluenesulfonic acid 3.7% 2% 3.7% 2% Dimethyl sulfoxide 6.7% 3% / / Dimethylformamide / / 6.7% 3% Anhydrous ethanol 1.5% 2% Water for injection / / 1.5% 2% Stability Stored at Stored at Stored at Stored at 4.degree. C. .+-. 4.degree. C. .+-. 4.degree. C. .+-. 4.degree. C. .+-. 2.degree. C. for 2.degree. C. for 2.degree. C. for 2.degree. C. for 10 days, 10 days, 10 days, 10 days, crystals crystals crystals crystals precip- precip- precip- precip- itated itated itated itated

[0044] The results show that the problem still exists when the formulation is adjusted while maintaining the same amount of suberic acid and p-Toluenesulfonic acid. We further confirmed that suberic acid and p-Toluenesulfonic acid is the cause of the stability problem.

[0045] We then replaced suberic acid and p-toluenesulfonic acid.

Experiment 2. Screening Experiment

[0046] To replace suberic acid and p-Toluenesulfonic acid with a variety of substances while keeping other conditions unchanged, the screening process is as follows:

Screening Experiment

TABLE-US-00008 [0047] Raw materials Formula A Formula B Formula C Formula D Formula E Formula F p-toluenesulfonamide 30% 40% 30% 40% 30% 40% PEG-400 33.5% 40% 33.5% 40% 33.5% 40% 1,2-propanediol 16.4% 10% 16.4% 10% 16.4% 10% Sebacic acid / / 8.2% 3% / / Azelaic acid / / / / 8.2% 3% Suberic acid 8.2% 3% / / / / 2-ethyl-1,3-hexanediol / / / / 3.7% 2% Hexanediol / / 3.7% 2% / / p-Toluenesulfonic acid 3.7% 2% / / / / Dimethyl sulfoxide 6.7% 3% 6.7% 3% 6.7% 3% Anhydrous ethanol 1.5% 2% 1.5% 2% 1.5% 2% Stability Stored Stored Stored Stored Stored Stored at 4.degree. C. .+-. at 4.degree. C. .+-. at 4.degree. C. .+-. at 4.degree. C. .+-. at 4.degree. C. .+-. at 4.degree. C. .+-. 2.degree. C. for 2.degree. C. for 2.degree. C. for 2.degree. C. for 2.degree. C. for 2.degree. C. for 10 days, 10 days, 10 days, 10 days, 10 days, 10 days, small small no no no no crystals crystals precipitates, precipitates, precipitates, precipitates, precipitated precipitated clear clear clear clear solution solution solution solution

[0048] The results show that the use of sebacic acid instead of suberic acid or the use of 2-ethyl-1,3-hexanediol instead of p-toluenesulfonic acid can solve the problems associated with the known method. However, as the dosage in the known formulation was unscientific, the preparation method involved an excessively long dissolving process, so the two formulations were combined and the amount of each raw material in the formula were determined.

Experiment 3. Screening Experiments for the Amount of Raw Materials.

TABLE-US-00009 [0049] Raw Materials Formula I Formula II Formula III Formula IV Formula V Formula VI p-Toluenesulfonamide .sup. 30% 40% 30% 25% 30% 40% PEG-400 33.5% 40% 40% 35% 30% 30% 1,2-propanediol 16.4% 10% 5% 7% 8% 10% Sebacic acid 2% 2% 5% 4% 4% 2% 2-ethyl-1,3-hexanediol 8% 3% 10% 15% 15% 18% Dimethyl sulfoxide 6.7% 3% 5% 7% 5% 0 Anhydrous ethanol 1.5% 2% 5% 7% 8% 0 Dissolution time 8 min 10 min 4 min 3 min 5 min 3.8 min

[0050] Results show that, although all raw materials dissolved in Formula I and II, the time taken is relatively long while for Formula III-VI, raw materials dissolved faster and the final solution is clear and transparent.

[0051] In addition, the present invention is further compared with existing pharmaceutical injections.

[0052] Three samples were taken from each of Example 1, Example 5, Example 6, and Example 7 (i.e., Formulation III-VI) of the present invention (Labelled as Samples A-D), and three samples were taken from self-prepared Example 2 from Patent CN1073415C (Labelled as Sample E). All samples were stored at 4.degree. C..+-.2.degree. C. for 10 days and samples were observed at Day 0, Day 5, and Day 5 for crystal precipitation. Results are shown in Table 2:

TABLE-US-00010 TABLE 2 Comparison of crystal precipitation between Formulation III-VI of the present invention and CN1073415C Examples Sample 0 Day 5 Day 10 Day A1 Clear and Clear and Clear and transparent solution transparent solution transparent solution A2 Clear and Clear and Clear and transparent solution transparent solution transparent solution A3 Clear and Clear and Clear and transparent solution transparent solution transparent solution B1 Clear and Clear and Clear and transparent solution transparent solution transparent solution B2 Clear and Clear and Clear and transparent solution transparent solution transparent solution B3 Clear and Clear and Clear and transparent solution transparent solution transparent solution C1 Clear and Clear and Clear and transparent solution transparent solution transparent solution C2 Clear and Clear and Clear and transparent solution transparent solution transparent solution C3 Clear and Clear and Clear and transparent solution transparent solution transparent solution D1 Clear and Clear and Clear and transparent solution transparent solution transparent solution D2 Clear and Clear and Clear and transparent solution transparent solution transparent solution D3 Clear and Clear and Clear and transparent solution transparent solution transparent solution E1 Clear and Precipitation of fine Precipitation of transparent solution crystals crystals E2 Clear and Precipitation of fine Precipitation of transparent solution crystals crystals E3 Clear and Clear and Precipitation of fine transparent solution transparent solution crystals

[0053] As shown in the above table, Formulations III-VI of the present invention showed a significant increase in stability at 4.degree. C. compared to self-prepared CN 1073415C Example 2. The solution is clear and transparent after 10-day storage, and no crystal precipitated.

Accelerated Stability Experiment:

[0054] Samples were taken from Example 1, Example 5, Example 6 and Example 7 (i.e., Formulation III-VI) of the present invention (Labelled as Samples A-D), and self-prepared CN 1073415 C Example 2 (Labelled as Sample E), and stored at 40.degree. C.+2.degree. C., 75%.+-.5% RH. Relevant properties were assessed and results are shown in the below table:

TABLE-US-00011 Sample Test items Day 0 Month 1 Month 2 Month 3 Month 6 .A Visible foreign matter compliant compliant compliant compliant compliant Relevant Largest 0.058 0.031 0.074 0.078 0.096 Substance Single Impurity (%) Total 0.136 0.269 0.294 0.346 0.401 impurities (%) Content 99.77 99.57 99.17 98.08 98.05 B Visible foreign matter compliant compliant compliant compliant compliant Relevant Largest 0.058 0.078 0.079 0.086 0.097 Substance single impurity (%) Total 0.136 0.155 0.217 0.263 0.415 impurities (%) Content 99.77 99.61 99.56 98.70 98.57 C Visible foreign matter compliant compliant compliant compliant compliant Relevant Largest 0.058 0.042 0.050 0.070 0.088 Substance single impurity (%) Total 0.136 0.137 0.315 0.391 0.436 impurities (%) Content 99.77 99.67 99.49 99.18 98.87 D Visible foreign matter compliant compliant compliant compliant compliant Relevant Largest 0.058 0.046 0.074 0.082 0.085 Substance single impurity (%) Total 0.136 0.253 0.319 0.326 0.421 impurities (%) Content 99.77 99.27 99.10 98.88 98.81 E Visible foreign matter compliant compliant compliant compliant compliant Relevant Largest 0.058 0.067 0.119 0.131 0.168 Substance single impurity (%) Total 0.136 0.253 0.571 0.644 0.850 impurities (%) Content 99.77 99.46 99.06 98.59 97.36

[0055] As shown in the table above, Formulations III-IV of the present invention were significantly more stable after storage at 40.degree. C..+-.2.degree. C., 75%.+-.5% RH for 6 months compared to CH1073415C Example 2. The growth rates of single largest impurity and total impurities were slower, and there were fewer impurities. The degradation rates of the raw materials were also slower and there was less degradation for Formulations III-IV.

EXAMPLES

[0056] The following examples are included to further illustrate the present invention. It should not be regarded as a limitation on the present invention.

Example 1: Injection

TABLE-US-00012 [0057] Raw materials Percentage by weight p-Toluenesulfonamide 30% PEG-400 30% 1,2-propanediol 8% Sebacic acid 4% 2-ethyl-1,3-hexanediol 15% Dimethyl sulfoxide 5% Anhydrous Ethanol 8%

[0058] Preparation Method: [0059] 1) Putting a prescription amount of sulfonamide compound, PEG-400, and 2-ethyl-1,3-hexanediol into a container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution A, for later use; [0060] 2) Putting a prescription amount of sebacic acid and 1,2-propanediol, into a separate container, stirring slowly at 85.degree. C.-95.degree. C. to form a miscible solution, Solution B, for later use; [0061] 3) Mixing Solution A and Solution B while maintaining the temperature at 85.degree. C.-95.degree. C., and stirring to obtain a homogenous solution for later use; [0062] 4) Putting a prescription amount of dimethyl sulfoxide and a small amount of anhydrous ethanol into a container, stirring and mixing well to obtain a homogenous solution, Solution C, for later use; [0063] 5) Cooling the mixture of Solution A and Solution B to 60.degree. C., adding Solution C to the mixture, stirring and mixing well before cooling down to room temperature, adding the remaining amount of anhydrous ethanol and mixing well; [0064] 6) Filtering with 0.45 um microporous membrane, aliquoting into 5 ml ampoules and sealing the ampoules; and [0065] 7) Sterilizing at 121.degree. C. for 30 minutes.

[0066] The purpose of adding anhydrous ethanol in this formulation is to control the total volume of the final solution, and further control the total drug content in the formulation. Therefore, the amount of anhydrous ethanol to be added in Step 4 should be slightly less than the prescription amount.

Example 2. Comparative Safety Test

[0067] Using mice as animal model, Example 1 of the p-toluenesulfonamide injection was compared with CN 1073415 C Example 2 by administering a single intravenous injection at the tail vein for comparative toxicity test. Results are shown below:

[0068] Animal death rate is lower for Example 1. LD50 of CN 1073415C Example 2 is 11.30 mg/kg, (95% CI=9.47-13.13 mg/kg) LD50 of Example 1 of the present invention is 18.10 mg/kg (95% CI=15.2-21.0 mg/kg). After the improvement of the current invention, i.e. replacing p-toluenesulfonic acid with 2-ethyl-1,3-hexanediol, LD50 of the drug was increased by 60% (P<0.01), significantly reducing the toxicity of the injection.

Example 3. Efficacy Experiment

[0069] Example 1 of the present invention was tested on animals. Table 2 shows the effect of intramuscular injection of Example 1 on the development of lung cancer in mice.

TABLE-US-00013 TABLE 2 Effect of intramuscular injection of Example 1 on lung cancer development in mice No. of Body Average weight Dosage subjects weight of tumor Tumor-inhibiting Group Treatment (/kg/d .times. 10 d) Start End Start End (X .+-. SD) (g) rate (%) P value* 1 NS(contnol) 2.0 ml 11 11 20.7 27.9 3.52 .+-. 0.62 0.0 >0.05 Solvent 2.0 ml 11 11 20.5 25.1 2.91 .+-. 0.58 17.3 <0.01 (control) CTX 18.0 ml 11 11 20.8 25.0 1.82 .+-. 0.41 48.3 <0.01 Example 1 0.5 ml 11 11 21.9 25.4 1.81 .+-. 0.25 48.6 <0.01 Example 1 1.0 ml 11 11 21.3 25.2 1.46 .+-. 0.74 58.5 <0.01 Example 1 2.0 ml 11 11 20.2 24.0 1.25 .+-. 0.72 64.5 <0.01 2 NS(control) 2.0 ml 11 11 20.4 27.7 2.49 .+-. 0.58 0.0 >0.05 Solvent 2.0 ml 11 11 21.5 28.9 2.69 .+-. 0.56 -8.0 <0.01 (control) CTX 18.0 ml 11 11 21.6 28.8 1.54 .+-. 0.27 38.2 <0.01 Example 1 0.5 ml 11 11 21.9 25.6 2.15 .+-. 0.87 13.7 <0.01 Example 1 1.0 ml 11 11 21.0 25.8 2.00 .+-. 0.82 19.7 <0.01 Example 1 2.0 ml 11 11 21.6 25.6 1.59 .+-. 0.64 36.1 <0.01 3 NS(control) 2.0 ml 11 11 21.0 27.6 2.51 .+-. 0.46 0.0 >0.01 Solvent 2.0 ml 11 11 21.4 28.5 1.82 .+-. 0.74 27.5 <0.01 (control) CTX 18.0 ml 11 11 21.7 27.4 1.02 .+-. 0.35 59.4 <0.01 Example 1 0.5 ml 11 11 21.8 25.9 1.67 .+-. 0.95 33.5 <0.01 Example 1 1.0 ml 11 11 21.1 25.3 1.43 .+-. 0.74 43.0 <0.01 Example 1 2.0 ml 11 11 20.3 25.6 1.02 .+-. 0.57 59.4 <0.01

[0070] The results of the experiments showed that: [0071] 1) At 0.5 ml, 1.0 ml, 2 ml, and 2.0 ml/kg/d.times.10d, Example 1 has different levels of anti-tumor effect on mice transplanted lung tumor. Anti-tumor effect increases as dosage increases. [0072] 2) At 2.0 ml/kg/d.times.10d dosage, Example 1 has no anti-tumor effect on mice liver cancer and mice sarcoma S-180. [0073] 3) This demonstrates that the selected dosages of Example 1 have significant anti-tumor effect on mice lung cancer. [0074] 4) Injection of Example 1 has a high therapeutic index for mice cancer.

Example 4. Clinical Trials

[0075] Single-arm clinical trial of Example 1 injection (PTS injection) for local intra-tumoral injection in patients with severe airway obstruction due to central type lung cancer.

[0076] Clinical studies of PTS injection conducted by the Guangzhou Institute of Respiratory Diseases and 21 clinical research institutes approved by the Ministry of Health reached the following conclusions:

[0077] 89 patients were enrolled in the PTS clinical trial. Of these, 10 dropped out, 7 were excluded from analysis and used only for safety assessment (either because the subjects did not meet the inclusion criteria or the investigator had a violation of clinical protocols). A total of 72 patients were included in the efficacy analysis.

[0078] Among the 72 subjects aged between 23-79, 59 were males and 13 were females. Subject statistics based on their lung cancer stage: 46 cases in IV phase, 22 cases in stage IIIb, 4 cases in stage IIIa. Subject statistics based on the location of tumor, 6 cases of airway, 28 cases of left main bronchus, 30 cases of right main bronchus, and 8 cases of right intermediate bronchus.

Results on Efficacy,

[0079] 72 patients with central lung cancer after 2-4 weeks of treatment with PTS local target tumor injection:

[0080] Major efficacy index: objective response rate of intraluminal target lesion-according to RECIST: based on CT assessment, the objective response rate was 68.08% within 7 days from the last treatment, and 48.61% after 30 days from the exit period, respectively.

[0081] Major efficacy index: objective response rate of intraluminal target lesion-according to WHO standard: based on CT assessment, the objective response rate of intraluminal target lesion was 77.78% within 7 days from the last treatment, and 54.17% after 30 days from the exit period.

[0082] Major efficacy index: improvement rate on luminal tumor blockage, applying CT assessment, the improvement rate for obstruction due to luminal tumor is 70.45% within 7 days from the last treatment, and 70.47% after 30 days from the exit period.

[0083] Clinical benefit index: Compared with the baseline, the pulmonary function index FEV1 showed statistically significant improvement within 7 days after the last administration, with a rate of improvement of 34.72% and 18.06% at 30 days after the exit period; the total re-expansion rate for patients with atelectasis across different lobes was 44.44% (20/45). Arranged by the location with a descending re-expansion rate is as follows: 50.00% for the right middle lobe, 43.75% for the left lung, 42.11% for the left lower lobe, 38.46% for the right lower lobe, 35.00% for the left tongue lobe, and 25.00% for the right upper lobe.

[0084] Analysis of the overall efficacy showed that, the target lesions significantly shrink after intra-tumoral injection of PTS, with the objective response rate and luminal tumor obstruction improvement rate close to 70%; clinical benefit index FEV1 improved more than 30%, pulmonary re-expansion rate improved more than 40%. The indexes above directly showed that patients have reduced target lesions after PTS treatment, their airway obstruction improved, indicating that respiratory functions and life quality significantly improved after PTS treatment.

Results on Safety:

[0085] During the entire course of the clinical trial on the 89 subjects, none of them exhibited bone marrow suppression, gastrointestinal reactions or other commonly observed adverse reactions associated with systemic chemotherapy.

[0086] During the entire course of the clinical trial, vital signs, physical examination, blood examination, blood biochemical examination, immunology examination and electrocardiogram were monitored and showed no statistically significant changes.

[0087] This demonstrates that intra-tumoral injection of PTS had little systemic impact on the patients.

Example 5. Injection

TABLE-US-00014 [0088] Raw materials Percentage by weight p-Toluenesulfonamide 40% PEG-400 30% 1,2-propanediol 10% Sebacic acid 2% 2-ethyl-1,3-hexanediol 18%

Same method of preparation as Example 1.

Example 6. Injection

TABLE-US-00015 [0089] Raw materials Percentage by weight p-Toluenesulfonamide 30% PEG-400 40% 1,2-propanediol 5% Sebacic acid 5% 2-ethyl-1,3-hexanediol 10% Dimethyl sulfoxide 5% Anhydrous ethanol 5%

[0090] Same method of preparation as Example 1.

Example 7. Injection

TABLE-US-00016 [0091] Raw materials Percentage by weight P-Toluenesulfonamide 25% PEG-400 35% 1,2-propanediol 7% Sebacic acid 4% 2-ethyl-1,3-hexanediol 15% Dimethyl sulfoxide 7% Anhydrous ethanol 7%

[0092] Same method of preparation as Example 1.

* * * * *

File A Patent Application

  • Protect your idea -- Don't let someone else file first. Learn more.

  • 3 Easy Steps -- Complete Form, application Review, and File. See our process.

  • Attorney Review -- Have your application reviewed by a Patent Attorney. See what's included.