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|United States Patent||5,989,835|
|Dunlay , et al.||November 23, 1999|
The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.
|Inventors:||Dunlay; R. Terry (Pittsburgh, PA), Taylor; D. Lansing (Pittsburgh, PA)|
|Filed:||February 27, 1997|
|Current U.S. Class:||506/10 ; 435/288.3; 435/288.4; 435/29; 435/40.5; 435/40.51; 435/7.2; 435/7.21; 436/172; 436/546; 436/800; 436/809; 506/41; 977/795; 977/881; 977/920; 977/941; 977/942|
|Current International Class:||C12Q 1/68 (20060101); G01N 15/14 (20060101); G01N 033/533 ()|
|Field of Search:||435/7.2,7.21,29,40.5,40.51,288.3,288.4 436/546,172,800,809|
|5355215||October 1994||Schroeder et al.|
|5401629||March 1995||Harpold et al.|
|5436128||July 1995||Harpold et al.|
|5491084||February 1996||Chalfie et al.|
|5548661||August 1996||Price et al.|
|WO 87 02802||Jul., 1987||WO|
|WO 94 11841||May., 1994||WO|
|WO 95/07463||Mar., 1995||WO|
|WO 95/21191||Aug., 1995||WO|
|WO 96/23898||Aug., 1996||WO|
|WO 96/27675||Sep., 1996||WO|
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